Recombinant Human MAPK6 Protein (1-721 aa), His-SUMO-tagged

• Cat.No.: MAPK6-644H
• Product Overview: Recombinant Human MAPK6 Protein (1-721 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Full Length.

Specification

• Description: Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 98.7 kDa
• Protein length: 1-721 aa
• AA Sequence: MAEKFESLMNIHGFDLGSRYMDLKP LGCGGNGLVFSAVDNDCDKRVAIKK IVLTDPQSVKHALREIKIIRRLDHD NIVKVFEILGPSGSQLTDDVGSLTE LNSVYIVQEYMETDLANVLEQGPLL EEHARLFMYQLLRGLKYIHSANVLH RDLKPANLFINTEDLVLKIGDFGLA RIMDPHYSHKGHLSEGLVTKWYRSP RLLLSPNNYTKAIDMWAAGCIFAEM LTGKTLFAGAHELEQMQLILESIPV VHEEDRQELLSVIPVYIRNDMTEPH KPLTQLLPGISREALDFLEQILTFS PMDRLTAEEALSHPYMSIYSFPMDE PISSHPFHIEDEVDDILLMDETHSH IYNWERYHDCQFSEHDWPVHNNFDI DEVQLDPRALSDVTDEEEVQVDPRK YLDGDREKYLEDPAFDTNYSTEPCW QYSDHHENKYCDLECSHTCNYKTRS SSYLDNLVWRESEVNHYYEPKLIID LSNWKEQSKEKSDKKGKSKCERNGL VKAQIALEEASQQLAGKEREKNQGF DFDSFIAGTIQLSSQHEPTDVVDKL NDLNSSVSQLELKSLISKSVSQEKQ EKGMANLAQLEALYQSSWDSQFVSG GEDCFFINQFCEVRKDEQVEKENTY TSYLDKFFSRKEDTEMLETEPVEDG KLGERGHEEGFLNNSGEFLFNKQLE SIGIPQFHSPVGSPLKSIQATLTPS AMKSSPQIPHQTYSSILKHLN
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: MAPK6 mitogen-activated protein kinase 6 [ Homo sapiens ]
• Official Symbol: MAPK6
• Synonyms: MAPK6; PRKM6; ERK3; HsT17250; p97MAPK; ERK-3; MAPK 6; p97-MAPK; MAP kinase 6; DKFZp686F03189
• Gene ID: 5597
• mRNA Refseq: NM_002748
• Protein Refseq: NP_002739
• MIM: 602904
• UniProt ID: Q16659