Recombinant Human matrix metallopeptidase 28, His-tagged

• Cat.No.: MMP28-449H
• Product Overview: MMP28,123-520aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 123-520 a.a.
• Description: MMP28 protein of the matrix metalloproteinase (MMP) family is involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction and tissue remodeling, and disease processes, such as asthma and metastasis. MMP28 is a secreted enzyme that degrades casein. Its expression pattern suggests that it plays a role in tissue homeostasis and in wound repair. Transcript variants encoding different isoforms have been described. Recombinant human MMP28 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 47.3 kDa (421aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSFAKQGNK WYKQHLSYRL VNWPEHLPEP AVRGAVRAAF QLWSNVSALE FWEAPATGPA DIRLTFFQGD HNDGLGNAFD GPGGALAHAF LPRRGEAHFD QDERWSLSRR RGRNLFVVLA HEIGHTLGLT HSPAPRALMA PYYKRLGRDA LLSWDDVLAV QSLYGKPLGG SVAVQLPGKL FTDFETWDSY SPQGRRPETQ GPKYCHSSFD AITVDRQQQL YIFKGSHFWE VAADGNVSEP RPLQERWVGL PPNIEAAAVS LNDGDFYFFK GGRCWRFRGP KPVWGLPQLC RAGGLPRHPD AALFFPPLRR LILFKGARYY VLARGGLQVE PYYPRSLQDW GGIPEEVSGA LPRPDGSIIF FRDDRYWRLD QAKLQATTSG RWATELPWMG CWHANSGSAL F
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MMP28 matrix metallopeptidase 28 [ Homo sapiens ]
• Official Symbol: MMP28
• Synonyms: MMP28; matrix metallopeptidase 28; matrix metalloproteinase 28; matrix metalloproteinase-28; putative MMP28 gene product; EPILYSIN; MM28; MMP 25; MMP 28; matrix metalloproteinase-28; MMP25; MMP-28
• Gene ID: 79148
• mRNA Refseq: NM_001032278
• Protein Refseq: NP_001027449
• MIM: 608417
• UniProt ID: Q9H239
• Chromosome Location: 17q21.1
• Pathway: Matrix Metalloproteinases, organism-specific biosystem
• Function: calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding

Citation

A new generation of topical chronic wound treatments containing specific MMP inhibitors

Journal: Chronic Wound Care Management and Research    Data: 2014/9/1

Authors: Ravi Shrivastava, Claire Janicot, Pedro Neto

Article Snippet:Selection of MMPs involved in eCM destruction Based on literature review, all commercially available purified MMPs were tested.Selection of MMPs involved in eCM destruction Based on literature review, all commercially available purified MMPs were tested.. Purified recombinant human matrix MMP-1 (intestinal collagenase), MMP-2 (gelatinase), MMP-3 (stromelysin 1, progelatinase), MMP-7 (matrilysin, uterine), MMP-8 (neutrophil collagenase), MMP-9 (gelatinase B), MMP-10 (stromelysin-2), MMP-11 (stromelysin-3), MMP12 (macrophage elastase), MMP-13 (collagenase-3), MMP14 (membrane-inserted/MT1-MMP), MMP-15 (MTMMP2/ SMCP-2), MMP-16 (MMP-X2), MMP-17 (MT4-MMP), MMP-19 (MMP-RASI), MMP-20 (enamelysin), MMP-21, MMP-23 (CA-MMP), MMP-24 (MT5-MMP), MMP-26 (endometase/matrilysin), MMP-27 (MMpeptidase 27), and MMP-28 (epilysin) were purchased from Creative-Biomart (Shirley, NY, USA).. All experiments were conducted using a fixed MMP concentration of 10 μg MMP Eq/mL (equivalents of solutes per milliliter), which was selected on the basis of the median MMP concentration in chronic wound fluids.25 MMPs were then associated with each other to identify the group of epithelial or fibroblast cell matrix-destroying MMPs (VB-MMPs).All experiments were conducted using a fixed MMP concentration of 10 μg MMP Eq/mL (equivalents of solutes per milliliter), which was selected on..