Active Recombinant Human MCAM protein, His-tagged

• Cat.No.: MCAM-616H
• Product Overview: Active Recombinant Human MCAM protein(P43121)(Val24-Gly559) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 24-559 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human MUC18, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MUC18 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 60.8 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MCAM melanoma cell adhesion molecule [ Homo sapiens ]
• Official Symbol: MCAM
• Synonyms: MCAM; melanoma cell adhesion molecule; cell surface glycoprotein MUC18; CD146; Gicerin; MUC18; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
• Gene ID: 4162
• mRNA Refseq: NM_006500
• Protein Refseq: NP_006491
• MIM: 155735
• UniProt ID: P43121