Recombinant Human MCAM Protein, C-His-tagged

• Cat.No.: MCAM-099H
• Product Overview: Recombinant Human MCAM Protein with C-His tag was expressed in E. coli.

Specification

• Description: Melanoma cell adhesion molecule (MCAM, MUC18, CD146) is an immunoglobulin superfamily member originally described as a cell surface adhesion protein and marker of the progression and metastasis of melanoma. Expression of MCAM protein is seen in vascular endothelial cells, activated T lymphocytes, smooth muscle, and bone marrow stromal cells. Research studies demonstrate increased MCAM expression in endothelial cells from angiogenesis-related disorders, including inflammatory bowel disease, Crohn’s disease, rheumatoid arthritis, tumors, and chronic renal failure. MCAM-expressing human mesenchymal stromal cells (hMSC) in the hematopoietic microenvironment are responsible for maintaining the self-renewal of hematopoietic stem and progenitor cells (HSPC) through direct contact between hMSC and HSPC. Related studies suggest that activation of the Notch signaling pathway may also, in part, play a role in HSPC maintenance. Additional research indicates that MCAM may play a role in multiple sclerosis, an autoimmune inflammatory disease that affects central nervous system neurons. Endothelial MCAM within the blood-brain barrier act as adhesion receptors that permit lymphocytes to transmigrate across the barrier and produce the inflammatory lesions that characterize the disorder.
• Source: E. coli
• Species: Human
• Tag: C-His
• Molecular Mass: ~59 kDa
• AA Sequence: VPGEAEQPAPELVEVEVGSTALLKC GLSQSQGNLSHVDWFSVHKEKRTLI FRVRQGQGQSEPGEYEQRLSLQDRG ATLALTQVTPQDERIFLCQGKRPRS QEYRIQLRVYKAPEEPNIQVNPLGI PVNSKEPEEVATCVGRNGYPIPQVI WYKNGRPLKEEKNRVHIQSSQTVES SGLYTLQSILKAQLVKEDKDAQFYC ELNYRLPSGNHMKESREVTVPVFYP TEKVWLEVEPVGMLKEGDRVEIRCL ADGNPPPHFSISKQNPSTREAEEET TNDNGVLVLEPARKEHSGRYECQGL DLDTMISLLSEPQELLVNYVSDVRV SPAAPERQEGSSLTLTCEAESSQDL EFQWLREETGQVLERGPVLQLHDLK REAGGGYRCVASVPSIPGLNRTQLV NVAIFGPPWMAFKERKVWVKENMVL NLSCEASGHPRPTISWNVNGTASEQ DQDPQRVLSTLNVLVTPELLETGVE CTASNDLGKNTSILFLELVNLTTLT PDSNTTTGLSTSTASPHTRANSTST ERKLPEPESRG
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Concentration: ≥0.5 mg/mL
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: MCAM melanoma cell adhesion molecule [ Homo sapiens (human) ]
• Official Symbol: MCAM
• Synonyms: MCAM; melanoma cell adhesion molecule; cell surface glycoprotein MUC18; CD146; Gicerin; MUC18; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
• Gene ID: 4162
• mRNA Refseq: NM_006500
• Protein Refseq: NP_006491
• MIM: 155735
• UniProt ID: P43121