Active Recombinant Human MCAM protein, Fc-tagged

• Cat.No.: MCAM-615H
• Product Overview: Active Recombinant Human MCAM protein(Met 1-Gly 559)(NP_006491.2) was expressed in Human Cells with the C-terminal fused Fc region of human IgG1.

Specification

• Species: Human
• Source: Human Cells
• Tag: Fc
• Protein Length: 1-559 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 3 x 10E4 cells/well are added to human CD146 coated plates (0.5 μg/ml, 100 μl/well), approximately >30 % will adhere after one hour at 37 °C.
• Molecular Mass: The recombinant CD146/Fc chimera comprises 774 amino acids and predicts a molecular mass of 86.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 120-130 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MCAM melanoma cell adhesion molecule [ Homo sapiens ]
• Official Symbol: MCAM
• Synonyms: MCAM; melanoma cell adhesion molecule; cell surface glycoprotein MUC18; CD146; Gicerin; MUC18; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
• Gene ID: 4162
• mRNA Refseq: NM_006500
• Protein Refseq: NP_006491
• MIM: 155735
• UniProt ID: P43121