Recombinant Human MCAT Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MCAT-5054H
• Product Overview: MCAT MS Standard C13 and N15-labeled recombinant protein (NP_775738) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 42.9 kDa
• AA Sequence: MSVRVARVAWVRGLGASYRRGASSF PVPPPGAQGVAELLRDATGAEEEAP WAATERRMPGQCSVLLFPGQGSQVV GMGRGLLNYPRVRELYAAARRVLGY DLLELSLHGPQETLDRTVHCQPAIF VASLAAVEKLHHLQPSVIENCVAAA GFSVGEFAALVFAGAMEFAEGLYAV KIRAEAMQEASEAVPSGMLSVLGQP QSKFNFACLEAREHCKSLGIENPVC EVSNYLFPDCRVISGHQEALRFLQK NSSKFHFRRTRMLPVSGAFHTRLME PAVEPLTQALKAVDIKKPLVSVYSN VHGHRYRHPGHIHKLLAQQLVSPVK WEQTMHAIYERKKGRGFPQTFEVGP GRQLGAILKSCNMQAWKSYSAVDVL QTLEHVDLDPQEPPRTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MCAT malonyl-CoA-acyl carrier protein transacylase [ Homo sapiens (human) ]
• Official Symbol: MCAT
• Synonyms: MCAT; malonyl CoA:ACP acyltransferase (mitochondrial); malonyl-CoA-acyl carrier protein transacylase, mitochondrial; fabD; FASN2C; MCT; MT; NET62; mitochondrial malonyltransferase; [Acyl-carrier-protein] malonyltransferase; mitochondrial malonyl CoA:ACP acyltransferase; malonyl-CoA:acyl carrier protein transacylase, mitochondrial; MGC47838
• Gene ID: 27349
• mRNA Refseq: NM_173467
• Protein Refseq: NP_775738
• MIM: 614479
• UniProt ID: Q8IVS2