Recombinant Human MCM3AP Protein, His-tagged

• Cat.No.: MCM3AP-2430H
• Product Overview: Recombinant Human MCM3AP Protein (Phe33-Arg340) with a N-His tag was expressed in E. coli.

Specification

• Description: The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 35.9 kDa
  Accurate Molecular Mass: 39 kDa
• Protein length: Phe33-Arg340
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MCM3AP minichromosome maintenance complex component 3 associated protein [ Homo sapiens (human) ]
• Official Symbol: MCM3AP
• Synonyms: MCM3AP; minichromosome maintenance complex component 3 associated protein; MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein, minichromosome maintenance deficient (S. cerevisiae) 3 associated protein; 80 kDa MCM3-associated protein; GANP; KIAA0572; Map80; MCM3 import protein; germinal center-associated nuclear protein; minichromosome maintenance deficient 3-associated protein; MCM3 minichromosome maintenance deficient 3 associated protein; MAP80; FLJ44336; FLJ45306
• Gene ID: 8888
• mRNA Refseq: NM_003906
• Protein Refseq: NP_003897
• MIM: 603294
• UniProt ID: O60318