Recombinant Human MCM4 protein, His-tagged

• Cat.No.: MCM4-3275H
• Product Overview: Recombinant Human MCM4 protein(515-859 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 515-859 aa
• AA Sequence: SKSQLLQYVYNLVPRGQYTSGKGSS AVGLTAYVMKDPETRQLVLQTGALV LSDNGICCIDEFDKMNESTRSVLHE VMEQQTLSIAKAGIICQLNARTSVL AAANPIESQWNPKKTTIENIQLPHT LLSRFDLIFLMLDPQDEAYDRRLAH HLVALYYQSEEQAEEELLDMAVLKD YIAYAHSTIMPRLSEEASQALIEAY VDMRKIGSSRGMVSAYPRQLESLIR LAEAHAKVRLSNKVEAIDVEEAKRL HREALKQSATDPRTGIVDISILTTG MSATSRKRKEELAEALKKLILSKGK TPALKYQQLFEDIRGQSDIAITKDM FEEALRALADDDFLTVTGKT
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: MCM4 minichromosome maintenance complex component 4 [ Homo sapiens ]
• Official Symbol: MCM4
• Synonyms: MCM4; minichromosome maintenance complex component 4; CDC21, MCM4 minichromosome maintenance deficient 4 (S. cerevisiae); DNA replication licensing factor MCM4; CDC54; hCdc21; MGC33310; P1 Cdc21; CDC21 homolog; minichromosome maintenance deficient 4; homolog of S. pombe cell devision cycle 21; MCM4 minichromosome maintenance deficient 4; CDC21; P1-CDC21
• Gene ID: 4173
• mRNA Refseq: NM_005914
• Protein Refseq: NP_005905
• MIM: 602638
• UniProt ID: P33991