| Description : |
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
96.4 kDa |
| AA Sequence : |
MSSPASTPSRRGSRRGRATPAQTPR SEDARSSPSQRRRGEDSTSTGELQP MPTSPGVDLQSPAAQDVLFSSPPQM HSSAIPLDFDVSSPLTYGTPSSRVE GTPRSGVRGTPVRQRPDLGSAQKGL QVDLQSDGAAAEDIVASEQSLGQKL VIWGTDVNVAACKENFQRFLQRFID PLAKEEENVGIDITEPLYMQRLGEI NVIGEPFLNVNCEHIKSFDKNLYRQ LISYPQEVIPTFDMAVNEIFFDRYP DSILEHQIQVRPFNALKTKNMRNLN PEDIDQLITISGMVIRTSQLIPEMQ EAFFQCQVCAHTTRVEMDRGRIAEP SVCGRCHTTHSMALIHNRSLFSDKQ MIKLQESPEDMPAGQTPHTVILFAH NDLVDKVQPGDRVNVTGIYRAVPIR VNPRVSNVKSVYKTHIDVIHYRKTD AKRLHGLDEEAEQKLFSEKRVELLK ELSRKPDIYERLASALAPSIYEHED IKKGILLQLFGGTRKDFSHTGRGKF RAEINILLCGDPGTSKSQLLQYVYN LVPRGQYTSGKGSSAVGLTAYVMKD PETRQLVLQTGALVLSDNGICCIDE FDKMNESTRSVLHEVMEQQTLSIAK AGIICQLNARTSVLAAANPIESQWN PKKTTIENIQLPHTLLSRFDLIFLM LDPQDEAYDRRLAHHLVALYYQSEE QAEEELLDMAVLKDYIAYAHSTIMP RLSEEASQALIEAYVDMRKIGSSRG MVSAYPRQLESLIRLAEAHAKVRLS NKVEAIDVEEAKRLHREALKQSATD PRTGIVDISILTTGMSATSRKRKEE LAEALKKLILSKGKTPALKYQQLFE DIRGQSDIAITKDMFEEALRALADD DFLTVTGKTVRLLTRTRPLEQKLIS EEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
