| Description : |
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
92.9 kDa |
| AA Sequence : |
MDLAAAAEPGAGSQHLEVRDEVAEK CQKLFLDFLEEFQSSDGEIKYLQLA EELIRPERNTLVVSFVDLEQFNQQL STTIQEEFYRVYPYLCRALKTFVKD RKEIPLAKDFYVAFQDLPTRHKIRE LTSSRIGLLTRISGQVVRTHPVHPE LVSGTFLCLDCQTVIRDVEQQFKYT QPNICRNPVCANRRRFLLDTNKSRF VDFQKVRIQETQAELPRGSIPRSLE VILRAEAVESAQAGDKCDFTGTLIV VPDVSKLSTPGARAETNSRVSGVDG YETEGIRGLRALGVRDLSYRLVFLA CCVAPTNPRFGGKELRDEEQTAESI KNQMTVKEWEKVFEMSQDKNLYHNL CTSLFPTIHGNDEVKRGVLLMLFGG VPKTTGEGTSLRGDINVCIVGDPST AKSQFLKHVEEFSPRAVYTSGKASS AAGLTAAVVRDEESHEFVIEAGALM LADNGVCCIDEFDKMDVRDQVAIHE AMEQQTISITKAGVKATLNARTSIL AAANPISGHYDRSKSLKQNINLSAP IMSRFDLFFILVDECNEVTDYAIAR RIVDLHSRIEESIDRVYSLDDIRRY LLFARQFKPKISKESEDFIVEQYKH LRQRDGSGVTKSSWRITVRQLESMI RLSEAMARMHCCDEVQPKHVKEAFR LLNKSIIRVETPDVNLDQEEEIQME VDEGAGGINGHADSPAPVNGINGYN EDINQESAPKASLRLGFSEYCRISN LIVLHLRKVEEEEDESALKRSELVN WYLKEIESEIDSEEELINKKRIIEK VIHRLTHYDHVLIELTQAGLKGSTE GSESYEEDPYLVVNPNYLLEDTRTR PLEQKLISEEDLAANDILDYKDDDD KV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
