Human MCM6 Knockdown Cell Lysate

• Cat.No.: MCM6-238HKCL
• Product Overview: WB-validated MCM6 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 93 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg MCM6 KD HeLa cell lysate
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: MCM6 minichromosome maintenance complex component 6 [ Homo sapiens (human) ]
• Official Symbol: MCM6
• Synonyms: MCM6; minichromosome maintenance complex component 6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae), minichromosome maintenance deficient (mis5, S. pombe) 6, minichromosome maintenance deficient 6 homolog (S. cerevisiae); DNA replication licensing factor MCM6; Mis5; MIS5 homolog (S.pombe); MIS5 homolog; minichromosome maintenance deficient 6 homolog; minichromosome maintenance deficient (mis5, S. pombe) 6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); P105MCM; MCG40308
• Gene ID: 4175
• mRNA Refseq: NM_005915
• Protein Refseq: NP_005906
• MIM: 601806
• UniProt ID: Q14566