Recombinant Human MDK Protein, His-tagged

• Cat.No.: MDK-2431H
• Product Overview: Recombinant Human MDK Protein (Ala22-Asp143) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala22-Asp143
• Description: This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.0 kDa
  Accurate Molecular Mass: 20 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MDK midkine (neurite growth-promoting factor 2) [ Homo sapiens (human) ]
• Official Symbol: MDK
• Synonyms: MDK; midkine (neurite growth-promoting factor 2); NEGF2; midkine; FLJ27379; MK; ARAP; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting protein; neurite outgrowth-promoting factor 2
• Gene ID: 4192
• mRNA Refseq: NM_001012333
• Protein Refseq: NP_001012333
• MIM: 162096
• UniProt ID: P21741