Recombinant Human ME2 protein, His & T7-tagged

• Cat.No.: ME2-1545H
• Product Overview: Recombinant Human ME2 aa. (Gly220~Ala426 (Accession # P23368)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Gly220~Ala426
• Description: This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.8kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 7

Gene Information

• Gene Name: ME2 malic enzyme 2 [ Homo sapiens (human) ]
• Official Symbol: ME2
• Synonyms: ME2; malic enzyme 2; ODS1; NAD-dependent malic enzyme, mitochondrial; NAD-ME; malate dehydrogenase (oxaloacetate-decarboxylating); malic enzyme 2, NAD(+)-dependent, mitochondrial; pyruvic-malic carboxylase
• Gene ID: 4200
• mRNA Refseq: NM_001168335.1
• Protein Refseq: NP_001161807.1
• UniProt ID: P23368