Recombinant Human MECP2 protein, His-tagged

• Cat.No.: MECP2-66H
• Product Overview: Recombinant human MECP2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: MECP2, also known as methyl-CpG-binding protein 2 isoform 1, is found in all cells in the body, including the brain, acting as a transcriptional repressor and activator. In the brain, it is found in high concentrations in neurons and is associated with maturation of the central nervous system (CNS) and in forming synaptic contacts.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 53.2kDa (492aa)50-70kDa (SDS-PAGE under reducing conditions)
• AA Sequence: MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEEKE GKHEPVQPSA HHSAEPAEAG KAETSEGSGS APAVPEASAS PKQRRSIIRDRGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPKSPKAPGTGRGRGR PKGSGTTRPK AATSEGVQVK RVLEKSPGKL LVKMPFQTSP GGKAEGGGAT TSTQVMVIKR PGRKRKAEAD PQAIPKKRGRKPGSVVAAAA AEAKKKAVKE SSIRSVQETV LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS PKGRSSSASSPPKKEHHHHH HHSESPKAPV PLLPPLPPPP PEPESSEDPT SPPEPQDLSS SVCKEEKMPR GGSLESDGCP KEPAKTQPAV ATAATAAEKYKHRGEGERKD IVSSSMPRPN REEPVDSRTP VTERVSHHHH HH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS – PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MECP2 methyl CpG binding protein 2 (Rett syndrome) [ Homo sapiens ]
• Official Symbol: MECP2
• Synonyms: MECP2; methyl CpG binding protein 2 (Rett syndrome); mental retardation, X linked 16 , mental retardation, X linked 79 , MRX16, MRX79, RTT; methyl-CpG-binding protein 2; meCp-2 protein; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13; DKFZp686A24160
• Gene ID: 4204
• mRNA Refseq: NM_001110792
• Protein Refseq: NP_001104262
• MIM: 300005
• UniProt ID: P51608
• Chromosome Location: Xq28
• Function: DNA binding; double-stranded methylated DNA binding; protein N-terminus binding; protein binding; protein domain specific binding; transcription corepressor activity