Active Recombinant Human MET Protein, His & Avi-tagged, Biotinylated

• Cat.No.: MET-195H
• Product Overview: Biotinylated Active Recombinant Human MET Protein(P08581-1)(Glu25-Thr932) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 25-932 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22um filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human HGF R, His-Avi Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
• Molecular Mass: The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MET met proto-oncogene (hepatocyte growth factor receptor) [ Homo sapiens ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene (hepatocyte growth factor receptor); hepatocyte growth factor receptor; HGFR; RCCP2; SF receptor; HGF receptor; oncogene MET; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; AUTS9; c-Met
• Gene ID: 4233
• mRNA Refseq: NM_000245
• Protein Refseq: NP_000236
• MIM: 164860
• UniProt ID: P08581