Recombinant Human Methionine Sulfoxide Reductase A, His-tagged

• Cat.No.: MSRA-2407H
• Product Overview: Recombinant humanMSRA protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: MSRA (methioninesulfoxide reductase A ) belongs to the MsrA Met sulfoxide reductase family.This enzyme has an important function as a repair enzyme for proteins thathave been inactivated by oxidation. It catalyzes the reversibleoxidation-reduction of methionine sulfoxide in proteins to methionine. Inenzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide,and H2O, whereas its two products are peptide-L-methionine (R)-S-oxide andthioredoxin
• Form: Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
• Molecular Weight: 26.2kDa (237aa),confirmed by MALDI-TOF
• Purity: > 90% by SDS -PAGE
• Concentration: 0.5mg/ml (determinedby Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMGNSAS NIVSPQEALP GRKEQTPVAA KHHVNGNRTV EPFPEGTQMA VFGMGCFWGAERKFWVLKGV YSTQVGFAGG YTSNPTYKEV CSEKTGHAEV VRVVYQPEHM SFEELLKVFW ENHDPTQGMRQGNDHGTQYR SAIYPTSAKQ MEAALSSKEN YQKVLSEHGF GPITTDIREG QTFYYAEDYH QQYLSKNPNGYCGLGGTGVS CPVGIKK
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Officialsymbol: MSRA

Gene Information

• Gene Name: MSRA methionine sulfoxidereductase A [Homo sapiens]
• Synonyms: methioninesulfoxide reductase A; EC 1.8.4.11; Peptide Met(O) reductase; cytosolicmethionine-S-sulfoxide reductase; Peptide-methionine (S)-S-oxide reductase;peptide met (O) reductase; Protein-methionine-S-oxide reductase; peptidemethionine sulfoxide reductase; PMSR; mitochondrial peptide methioninesulfoxide reductase; OTTHUMP00000115600; OTTHUMP00000224855;OTTHUMP00000224856; OTTHUMP00000224858; OTTHUMP00000229541;OTTHUMP00000230073
• Gene ID: 4482
• mRNA Refseq: NM_001135670
• Protein Refseq: NP_001129142
• MIM: 601250
• UniProt ID: Q9UJ68
• Chromosome Location: 8p23.1
• Function: oxidoreductaseactivity; peptide-methionine-(S)-S-oxide reductase activity