| Description : |
RNA N6-methyltransferase that methylates adenosine residues of a subset of RNAs and plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. In presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, leading to intron retention and preventing MAT2A mRNA splicing. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, leading to stimulate splicing of the MAT2A retained intron, and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs. Specifically binds the 3'-end of the MALAT1 long non-coding RNA. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
63.6 kDa |
| AA Sequence : |
MALSKSMHARNRYKDKPPDFAYLAS KYPDFKQHVQINLNGRVSLNFKDPE AVRALTCTLLREDFGLSIDIPLERL IPTVPLRLNYIHWVEDLIGHQDSDK STLRRGIDIGTGASCIYPLLGATLN GWYFLATEVDDMCFNYAKKNVEQNN LSDLIKVVKVPQKTLLMDALKEESE IIYDFCMCNPPFFANQLEAKGVNSR NPRRPPPSSVNTGGITEIMAEGGEL EFVKRIIHDSLQLKKRLRWYSCMLG KKCSLAPLKEELRIQGVPKVTYTEF CQGRTMRWALAWSFYDDVTVPSPPS KRRKLEKPRKPITFVVLASVMKELS LKASPLRSETAEGIVVVTTWIEKIL TDLKVQHKRVPCGKEEVSLFLTAIE NSWIHLRRKKRERVRQLREVPRAPE DVIQALEEKKPTPKESGNSQELARG PQERTPCGPALREGEAAAVEGPCPS QESLSQEENPEPTEDERSEEKGGVE VLENCQGSSNGAQDQEASEQFGSPV AERGKRLPGVAGQYLFKCLINVKKE VDDALVEMHWVEGQNRDLMNQLCTY IRNQIFRLVAVNTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
