Active Recombinant Human MFAP4 protein, His-Flag-tagged, Low Endotoxin

• Cat.No.: MFAP4-4532H
• Product Overview: Recombinant Human MFAP4 protein(P55083-1)(Val22-Ala255), fused with N-terminal His and Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 22-255 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human MFAP4, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 90% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MFAP4 microfibrillar-associated protein 4 [ Homo sapiens ]
• Official Symbol: MFAP4
• Synonyms: MFAP4; microfibrillar-associated protein 4; microfibril-associated glycoprotein 4; microfibril associated glycoprotein 4
• Gene ID: 4239
• mRNA Refseq: NM_001198695
• Protein Refseq: NP_001185624
• MIM: 600596
• UniProt ID: P55083