Recombinant Human MGAT5 Protein, His tagged

• Cat.No.: MGAT5-04H
• Product Overview: Recombinant Human N-Acetylglucosaminyltransferase V/MGAT5 (Leu189-Leu741) with a C-terminal 10-His tag was expressed in mammalian cells.

Specification

• Species: Human
• Source: Mammalian cells
• Protein Length: 189-741 aa
• Description: The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies.
• Molecular Mass: 65 kDa
• Bio-activity: Measured by its ability to transfer N-Acetyl-alpha-D-glucosamine from UDP-N-Acetyl-alpha -D-glucosamine to a biantennary N-linked core pentasaccharide in a CD39L3 coupled assay. The specific activity is > 10 pmol/min/μg.
• N-terminal Sequence Analysis: Leu189
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl and EDTA.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: MGAT5 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase [ Homo sapiens (human) ]
• Official Symbol: MGAT5
• Synonyms: MGAT5; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase; GNT-V; GNT-VA; MGAT5A; glcNAc-T V; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; N-acetylglucosaminyl-transferase V; alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase V; mannoside acetylglucosaminyltransferase 5; mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase; EC 2.4.1.155
• Gene ID: 4249
• mRNA Refseq: NM_002410
• Protein Refseq: NP_002401
• MIM: 601774
• UniProt ID: Q09328