Recombinant Human MGEA5 protein, His-tagged

• Cat.No.: MGEA5-612H
• Product Overview: Recombinant Human MGEA5 aa. (Ser6~Lys240) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser6~Lys240
• Description: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications.
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 5.2.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MGEA5 meningioma expressed antigen 5 (hyaluronidase) [ Homo sapiens (human) ]
• Official Symbol: MGEA5
• Synonyms: MGEA5; meningioma expressed antigen 5 (hyaluronidase); OGA; MEA5; NCOAT; protein O-GlcNAcase; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; beta-hexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase
• Gene ID: 10724
• mRNA Refseq: NM_001142434.1
• Protein Refseq: NP_001135906.1
• UniProt ID: O60502