Recombinant Human MID2 Protein, His-tagged

• Cat.No.: MID2-2443H
• Product Overview: Recombinant Human MID2 Protein (Ser7-Glu258) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.1 kDa
  Accurate Molecular Mass: 32 kDa
• Protein length: Ser7-Glu258
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: MID2 midline 2 [ Homo sapiens (human) ]
• Official Symbol: MID2
• Synonyms: MID2; midline 2; probable E3 ubiquitin-protein ligase MID2; FXY2; RNF60; TRIM1; midin 2; midin-2; midline-2; midline defect 2; RING finger protein 60; tripartite motif protein 1; tripartite motif-containing protein 1; FLJ37715; FLJ41813
• Gene ID: 11043
• mRNA Refseq: NM_012216
• Protein Refseq: NP_036348
• MIM: 300204
• UniProt ID: Q9UJV3