Active Recombinant Human MIF protein

Species :	Human
Source :	E.coli
Tag :	Non
Protein Length :	1-115 aa
Form :	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. with 5% trehalose, 0.02% Tween-80.
Bio-activity :	Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
Molecular Mass :	Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids.
AASequence :	MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA
Endotoxin :	Less than 1 EU/µg of rHuMIF as determined by LAL method.
Purity :	≥95 by SDS-PAGE.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) [ Homo sapiens ]
Official Symbol	MIF
Synonyms	MIF; macrophage migration inhibitory factor (glycosylation-inhibiting factor); GLIF; macrophage migration inhibitory factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; MMIF;
Gene ID	4282
mRNA Refseq	NM_002415
Protein Refseq	NP_002406
MIM	153620
UniProt ID	P14174