Recombinant Human MIOX Protein, GST-tagged

• Cat.No.: MIOX-888H
• Product Overview: Recombinant human MIOX protein (1-285 aa encoded) with GST tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-285 a.a.
• Form: Powder
• AA Sequence: MKVTVGPDPSLVYRPDVDPEVAKDKASFRNYTSGPLLDRVFTTYKLMHTHQTVDFVRSKHAQFGGFSYKKMTVMEAVDLLDGLVDESDPDVDFPNSFHAFQTAEGIRKAHPDKDWFHLVGLLHDLGKVLALFGEPQWAVVGDTFPVGCRPQASVVFCDSTFQDNPDLQDPRYSTELGMYQPHCGLDRVLMSWGHDEYMYQVMKFNKFSLPPEAFYMIRFHSFYPWHTGRDYQQLCSQQDLAMLPWVREFNKFDLYTKCPDLPDVDKLRPYYQGLIDKYCPGILSW
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Notes: FOR IN VITRO RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR ANIMALS.
• Storage: Store for up to 12 months at -20 centigrade to -80 centigrade as lyophilized powder.
  Short-term storage: Store at 2-8 centigrade for (1-2 weeks).
  Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Storage Buffer: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.

Gene Information

• Gene Name: MIOX myo-inositol oxygenase [ Homo sapiens (human) ]
• Official Symbol: MIOX
• Synonyms: MIOX; myo-inositol oxygenase; ALDRL6; inositol oxygenase; MI oxygenase; aldehyde reductase (aldose reductase) like 6; aldehyde reductase-like 6; kidney-specific protein 32; renal-specific oxidoreductase; EC 1.13.99.1
• Gene ID: 55586
• mRNA Refseq: NM_017584
• Protein Refseq: NP_060054
• MIM: 606774
• UniProt ID: Q9UGB7