Recombinant Human mitochondrial ribosomal protein L2, His-tagged

• Cat.No.: MRPL2-681H
• Product Overview: MRPL2, 84-202aa Human, His tag, E.coli

Specification

• Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL2 is a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. Recombinant human MRPL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Phosphate buffer (pH 8.0) containing 1mM EDTA, 50% glycerol, 2mM DTT.
• Molecular Mass: 15.5 kDa (142aa) confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR YDPCRSADIA LVAGGSRKRW IIATENMQAG DTILNSNHIG RMAVAAREGD AHPLGALPVG TLINNVESEP GR
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Protein length: 84-202

Gene Information

• Gene Name: MRPL2 mitochondrial ribosomal protein L2 [ Homo sapiens ]
• Official Symbol: MRPL2
• Synonyms: MRPL2; mitochondrial ribosomal protein L2; RPML14; MRP-L14
• Gene ID: 65007