Recombinant Human MLH1 Protein, GST-tagged

• Cat.No.: MLH1-5385H
• Product Overview: Human MLH1 full-length ORF ( AAH06850, 1 a.a. - 756 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 108.9 kDa
• AA Sequence: MSFVAGVIRRLDETVVNRIAAGEVI QRPANAIKEMIENCLDAKSTSIQVI VKEGGLKLIQIQDNGTGIRKEDLDI VCERFTTSKLQSFEDLASISTYGFR GEALASISHVAHVTITTKTADGKCA YRASYSDGKLKAPPKPCAGNQGTQI TVEDLFYNIATRRKALKNPSEEYGK ILEVVGRYSVHNAGISFSVKKQGET VADVRTLPNASTVDNIRSIFGNAVS RELIEIGCEDKTLAFKMNGYISNAN YSVKKCIFLLFINHRLVESTSLRKA IETVYAAYLPKNTHPFLYLSLEISP QNVDVNVHPTKHEVHFLHEESILER VQQHIESKLLGSNSSRMYFTQTLLP GLAGPSGEMVKSTTSLTSSSTSGSS DKVYAHQMVRTDSREQKLDAFLQPL SKPLSSQPQAIVTEDKTDISSGRAR QQDEEMLELPAPAEVAAKNQSLEGD TTKGTSEMSEKRGPTSSNPRKRHRE DSDVEMVEDDSRKEMTAACTPRRRI INLTSVLSLQEEINEQGHEVLREML HNHSFVGCVNPQWALAQHQTKLYLL NTTKLSEELFYQILIYDFANFGVLR LSEPAPLFDLAMLALDSPESGWTEE DGPKEGLAEYIVEFLKKKAEMLADY FSLEIDEEGNLIGLPLLIDNYVPPL EGLPIFILRLATEVNWDEEKECFES LSKECAMFYSIRKQYISEESTLSGQ QSEVPGSIPNSWKWTVEHIVYKALR SHILPPKHFTEDGNILQLANLPDLY KVFERC
• Applications: Antibody Production
  Functional Study
  Compound Screening
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) [ Homo sapiens ]
• Official Symbol: MLH1
• Synonyms: MLH1; mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); COCA2, mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2); DNA mismatch repair protein Mlh1; FCC2; HNPCC; HNPCC2; COCA2; hMLH1; MGC5172
• Gene ID: 4292
• mRNA Refseq: NM_000249
• Protein Refseq: NP_000240
• MIM: 120436
• UniProt ID: P40692