Recombinant Human MMP17 protein

• Cat.No.: MMP17-27H
• Product Overview: Recombinant Human MMP17 was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigradefor one month. Aliquot and store at -80 centigradefor 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: MMP17 matrix metallopeptidase 17 (membrane-inserted) [ Homo sapiens ]
• Official Symbol: MMP17
• Synonyms: MMP17; matrix metallopeptidase 17 (membrane-inserted); matrix metalloproteinase 17 (membrane inserted); matrix metalloproteinase-17; MT4 MMP; MMP-17; MT4MMP; MTMMP4; MT-MMP 4; membrane-type matrix metalloproteinase 4; membrane-type-4 matrix metalloproteinase; matrix metalloproteinase 17 (membrane-inserted); MT4-MMP
• Gene ID: 4326
• mRNA Refseq: NM_016155
• Protein Refseq: NP_057239
• MIM: 602285
• UniProt ID: Q9ULZ9
• Chromosome Location: 12q24.3
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem
• Function: calcium ion binding; enzyme activator activity; metalloendopeptidase activity; peptidase activity; zinc ion binding

Citation

A new generation of topical chronic wound treatments containing specific MMP inhibitors

Journal: Chronic Wound Care Management and Research    Data: 2014/9/1

Authors: Ravi Shrivastava, Claire Janicot, Pedro Neto

Article Snippet:Selection of MMPs involved in eCM destruction Based on literature review, all commercially available purified MMPs were tested.Selection of MMPs involved in eCM destruction Based on literature review, all commercially available purified MMPs were tested.. Purified recombinant human matrix MMP-1 (intestinal collagenase), MMP-2 (gelatinase), MMP-3 (stromelysin 1, progelatinase), MMP-7 (matrilysin, uterine), MMP-8 (neutrophil collagenase), MMP-9 (gelatinase B), MMP-10 (stromelysin-2), MMP-11 (stromelysin-3), MMP12 (macrophage elastase), MMP-13 (collagenase-3), MMP14 (membrane-inserted/MT1-MMP), MMP-15 (MTMMP2/ SMCP-2), MMP-16 (MMP-X2), MMP-17 (MT4-MMP), MMP-19 (MMP-RASI), MMP-20 (enamelysin), MMP-21, MMP-23 (CA-MMP), MMP-24 (MT5-MMP), MMP-26 (endometase/matrilysin), MMP-27 (MMpeptidase 27), and MMP-28 (epilysin) were purchased from Creative-Biomart (Shirley, NY, USA).. All experiments were conducted using a fixed MMP concentration of 10 μg MMP Eq/mL (equivalents of solutes per milliliter), which was selected on the basis of the median MMP concentration in chronic wound fluids.25 MMPs were then associated with each other to identify the group of epithelial or fibroblast cell matrix-destroying MMPs (VB-MMPs).All experiments were conducted using a fixed MMP concentration of 10 μg MMP Eq/mL (equivalents of solutes per milliliter), which was selected on..