Recombinant Human MMP9 protein, His-tagged

• Cat.No.: MMP9-429H

Specification

• Product overview: Recombinant Human MMP9 (P14780) aa. (Gly213~Met422) fused with His tag was produced in Yeast cells.
• Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Protein length: Gly213~Met422
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB. If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: MMP9 matrix metallopeptidase 9 [ Homo sapiens (human) ]
• Official Symbol: MMP9
• Synonyms: MMP9; matrix metallopeptidase 9; GELB; CLG4B; MMP-9; MANDP2; macrophage gelatinase; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); type V collagenase
• Gene ID: 4318
• mRNA Refseq: NM_004994.2
• Protein Refseq: NP_004985.2
• UniProt ID: P14780

Reviews

05/03/2020

The superior quality of this recombined protein has significantly enhanced the precision of our research outcomes.

08/15/2019

Fast shipping! Ordered this product, and it arrived well-packaged and ready for use in our experiments.

01/21/2018

Customer service went above and beyond to accommodate our specific needs, ensuring a smooth purchasing experience.

Q&As

What are the consequences of dysregulated MMP9 expression in cancer progression?

Dysregulated MMP9 expression is associated with cancer invasion and metastasis, as it enables tumor cells to degrade the extracellular matrix and invade surrounding tissues.

What is the impact of MMP9 polymorphisms on enzyme activity?

Polymorphisms in the MMP9 gene can influence enzyme activity, potentially affecting the individual's susceptibility to certain diseases and the progression of pathological conditions.

Can you explain the role of MMP9 in tissue remodeling at the molecular level?

MMP9 facilitates tissue remodeling by cleaving structural proteins in the extracellular matrix, such as collagen and gelatin, leading to changes in tissue architecture.

What is the role of MMP9 in the breakdown of the blood-brain barrier during neurological disorders?

MMP9 contributes to the breakdown of the blood-brain barrier by degrading tight junction proteins, potentially exacerbating neurological disorders and neuroinflammation.

How does MMP9 contribute to angiogenesis at the molecular level?

MMP9 plays a role in angiogenesis by promoting the release and activation of growth factors sequestered in the extracellular matrix, facilitating the formation of new blood vessels.