Recombinant Human Mononucleosomes (H3.3 K27M)

• Cat.No.: NUC-048
• Product Overview: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a methionine at position 27, expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a methionine at position 27, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of M for K at position 27 in human H3.1 and H3.3 is a dominant negative inhibitor of the EZH2 methytransferase complex PRC2 and is associated with pediatric brain cancer (DIPG).
• Form: Mononucleosomes (H3.3 K27M), Recombinant Human (100 µg total mass, DNA+protein, 54.6 µg protein weight, ) 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
• Molecular Mass: 199,844 Da
• Applications: Mononucleosomes (H3.3 K27M), Recombinant Human are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. Mononucleosomes (H3.3 K27M), Recombinant Human do not contain free DNA which could alter assayed activities.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.