| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a. 2-130(end)), H2B (a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Each H2B has a C-terminal Cys added as a biotinylation site. Total histone MW (before biotinylation) =113.8 kDa. |
| Form : |
20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol. |
| Molecular Mass : |
113.8 kDa |
| Applications : |
Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications. Since these nucleosomes were prepared in E |
| Notes : |
The concentration of this product is determined by Bradford and represents only the protein concentration. |
| Stability : |
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1-0.5%). |
| Storage : |
Stable at -80°C for at least 6 months from date of receipt, when stored as directed |
| Concentration : |
1.01 mg/ml |
