Recombinant Human MRP63 Protein, His-tagged

• Cat.No.: MRP63-2455H
• Product Overview: Recombinant Human MRP63 Protein (Met1-Ser102) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Ser102
• Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein which belongs to an undetermined ribosomal subunit and which seems to be specific to animal mitoribosomes. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 3p, 5q, 8q, 14q, and Y.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16 kDa
  Accurate Molecular Mass: 18 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: MRP63 mitochondrial ribosomal protein 63 [ Homo sapiens (human) ]
• Official Symbol: MRP63
• Synonyms: MRPL57; mitochondrial ribosomal protein L57; MRP63; bMRP63; ribosomal protein 63, mitochondrial; hMRP63; mitochondrial large ribosomal subunit protein mL63; mitochondrial ribosomal protein 63; mitochondrial ribosomal protein bMRP63
• Gene ID: 78988
• mRNA Refseq: NM_024026
• Protein Refseq: NP_076931
• MIM: 611997
• UniProt ID: Q9BQC6