Recombinant Human MRPL48 293 Cell Lysate

Cat.No. : MRPL48-4161HCL
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Description : Antigen standard for mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : MRPL48 mitochondrial ribosomal protein L48 [ Homo sapiens ]
Official Symbol : MRPL48
Synonyms : MRPL48; mitochondrial ribosomal protein L48; 39S ribosomal protein L48, mitochondrial; CGI 118; L48MT; CGI-118; HSPC290; MRP-L48; FLJ17047; FLJ99260; MGC13323;
Gene ID : 51642
mRNA Refseq : NM_016055
Protein Refseq : NP_057139
MIM : 611853
UniProt ID : Q96GC5
Chromosome Location : 11q13.4
Function : protein binding; structural constituent of ribosome;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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