Recombinant Human MSLN Protein, Fc-tagged

• Cat.No.: MSLN-383H
• Product Overview: Recombinant human MSLN protein with Fc tag was expressed in HEK293.

Specification

• Description: This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: 59.6 kDa
• Protein Length: 630
• AA Sequence: MALPTARPLLGSCGTPALGSLLFLL FSLGWVQPSRTLAGETGQEAAPLDG VLANPPNISSLSPRQLLGFPCAEVS GLSTERVRELAVALAQKNVKLSTEQ LRCLAHRLSEPPEDLDALPLDLLLF LNPDAFSGPQACTRFFSRITKANVD LLPRGAPERQRLLPAALACWGVRGS LLSEADVRALGGLACDLPGRFVAES AEVLLPRLVSCPGPLDQDQQEAARA ALQGGGPPYGPPSTWSVSTMDALRG LLPVLGQPIIRSIPQGIVAAWRQRS SRDPSWRQPERTILRPRFRREVEKT ACPSGKKAREIDESLIFYKKWELEA CVDAALLATQMDRVNAIPFTYEQLD VLKHKLDELYPQGYPESVIQHLGYL FLKMSPEDIRKWNVTSLETLKALLE VNKGHEMSPQAPRRPLPQVATLIDR FVKGRGQLDKDTLDTLTAFYPGYLC SLSPEELSSVPPSSIWAVRPQDLDT CDPRQLDVLYPKARLAFQNMNGSEY FVKIQSFLGGAPTEDLKALSQQNVS MDLATFMKLRTDAVLPLTVAEVQKL LGPHVEGLKAEERHRPVRDWILRQR QDDLDTLGLGLQGGIPNGYLVLDLS MQEALSGTPCLLGPGPVLTVLALLL ASTLA
• Purity: > 98%
• Applications: WB; ELISA; FACS; FC
• Stability: This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
• Reconstitution: Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.

Gene Information

• Gene Name: MSLN mesothelin [ Homo sapiens (human) ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP
• Gene ID: 10232
• mRNA Refseq: NM_001177355
• Protein Refseq: NP_001170826
• MIM: 601051
• UniProt ID: Q13421

Reviews

05/22/2022

Very satisfied, great product.

03/06/2022

Product is high-quality.

09/30/2021

Excellent for experiments, reliable.

Q&As

What is the primary role of MSLN in normal tissues?

MSLN is normally expressed in mesothelial cells, playing a role in cell adhesion.

How is MSLN expression altered in cancerous cells?

In cancer, MSLN expression is significantly upregulated.

In which types of cancer is MSLN overexpressed?

MSLN is overexpressed in mesothelioma, ovarian, and pancreatic cancers.

Are there any therapeutic strategies targeting MSLN in cancer treatment?

Therapies targeting MSLN include antibody-drug conjugates and CAR-T cell therapies.

How can MSLN levels be detected and measured in clinical settings?

MSLN levels can be assessed through immunohistochemistry and serum biomarker tests.

What is the prognostic significance of MSLN overexpression in cancer patients?

MSLN overexpression is often associated with poor prognosis in cancer patients.

How does MSLN contribute to cancer progression and metastasis?

MSLN promotes tumor cell proliferation, invasion, and resistance to apoptosis.