Active Recombinant Human MSLN protein, His-Avi-tagged, FITC-Labeled

• Cat.No.: MSLN-3527H
• Product Overview: FITC-Labeled Active Recombinant Human MSLN protein(Q13421-2)(Glu296-Gly580) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 296-580 aa
• Conjugation/Label: FITC
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Wavelength: Excitation Wavelength: 490 nm Emission Wavelength: 520 nm
• Bio-activity: FACS Analysis of Anti-MSLN CAR Expression. 293T cells were transfected with anti-MSLN-scFv and His tag. Cells were stained with 5μg/ml FITC-Labeled Human MSLN (296-580) , His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.
• Molecular Mass: The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Concentration: 0.1-1.0 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MSLN mesothelin [ Homo sapiens ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP
• Gene ID: 10232
• mRNA Refseq: NM_001177355
• Protein Refseq: NP_001170826
• MIM: 601051
• UniProt ID: Q13421