Recombinant Human MTHFD2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MTHFD2-5322H
• Product Overview: MTHFD2 MS Standard C13 and N15-labeled recombinant protein (NP_006627) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 37.9 kDa
• AA Sequence: MAATSLMSALAARLLQPAHSCSLRL RPFHLAAVRNEAVVISGRKLAQQIK QEVRQEVEEWVASGNKRPHLSVILV GENPASHSYVLNKTRAAAVVGINSE TIMKPASISEEELLNLINKLNNDDN VDGLLVQLPLPEHIDERRICNAVSP DKDVDGFHVINVGRMCLDQYSMLPA TPWGVWEIIKRTGIPTLGKNVVVAG RSKNVGMPIAMLLHTDGAHERPGGD ATVTISHRYTPKEQLKKHTILADIV ISAAGIPNLITADMIKEGAAVIDVG INRVHDPVTAKPKLVGDVDFEGVRQ KAGYITPVPGGVGPMTVAMLMKNTI IAAKKVLRLEEREVLKSKELGVATN TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MTHFD2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase [ Homo sapiens (human) ]
• Official Symbol: MTHFD2
• Synonyms: MTHFD2; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase; bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase; NMDMC
• Gene ID: 10797
• mRNA Refseq: NM_006636
• Protein Refseq: NP_006627
• MIM: 604887
• UniProt ID: P13995