Recombinant Human MUC16 Protein, His-tagged

• Cat.No.: MUC16-02H
• Product Overview: Recombinant Human MUC16 Protein(NP_078966.2), fused to His-tag, was expressed in HEK293.

Specification

• Description: This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes.
• Source: HEK293
• Species: Human
• Tag: His
• Form: PBS, pH 7.4.
• Molecular Mass: The protein has a calculated MW of 75 kDa.
• AA Sequence: MEKIPVSAFLLLVALSYTLAPGSRK FNTTERVLQGLLRPLFKNTSVGPLY SGCRLTLLRPEKDGEATGVDAICTH RPDPTGPGLDREQLYLELSQLTHSI TELGPYTLDRDSLYVNGFTHRSSVP TTSTGVVSEEPFTLNFTINNLRYMA DMGQPGSLKFNITDNVMQHLLSPLF QRSSLGARYTGCRVIALRSVKNGAE TRVDLLCTYLQPLSGPGLPIKQVFH ELSQQTHGITRLGPYSLDKDSLYLN GYNEPGPDEPPTTPKPATTFLPPLS EATTAMGYHLKTLTLNFTISNLQYS PDMGKGSATFNSTEGVLQHLLRPLF QKSSMGPFYLGCQLISLRPEKDGAA TGVDTTCTYHPDPVGPGLDIQQLYW ELSQLTHGVTQLGFYVLDRDSLFIN GYAPQNLSIRGEYQINFHIVNWNLS NPDPTSSEYITLLRDIQDKVTTLYK GSQLHDTFRFCLVTNLTMDSVLVTV KALFSSNLDPSLVEQVFLDKTLNAS FHWLGSTYQLVDIHVTEMESSVYQP TSSSSTQHFYLNFTITNLPYSQDKA QPGTTNYQRNKRNIEDALNQLFRNS SIKSYFSDCQVSTFRSVPNRHHTGV DSLCNFSPLARRVDRVAIYEEFLRM TRNGTQLQNFTLDRSSVLVDGYSPN RNEPLTGNSDLPHHHHHH
• Purity: >90%, by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.1 mg/ml

Gene Information

• Gene Name: MUC16 mucin 16, cell surface associated [ Homo sapiens (human) ]
• Official Symbol: MUC16
• Synonyms: CA125
• Gene ID: 94025
• mRNA Refseq: NM_024690.2
• Protein Refseq: NP_078966.2
• MIM: 606154
• UniProt ID: Q8WXI7