Recombinant Human MUSK Protein, Fc tagged

• Cat.No.: MUSK-48H
• Product Overview: Recombinant Human MUSK Protein (Leu24-Thr495) with Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 24-495aa
• Description: This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.
• Tag: C-Fc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. In the presence of Recombinant Human Agrin, immobilized Recombinant Human MuSK Fc Chimera (2 μg/mL at 100 μL/well) binds Recombinant Human LRP-4 with an ED50 of 5-50 ng/mL.
• Molecular Mass: 78 kDa
• Endotoxin: < 0.1 EU/μg by LAL.
• Purity: > 95 %, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• N-terminal Sequence Analysis: Leu24
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• References: 1. Valenzuela, D.M. et al. (1995) Neuron 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.

Gene Information

• Gene Name: MUSK muscle, skeletal, receptor tyrosine kinase [Homo sapiens (human)]
• Official Symbol: MUSK
• Synonyms: MUSK; muscle, skeletal, receptor tyrosine kinase; muscle, skeletal receptor tyrosine-protein kinase; muscle-specific kinase receptor; muscle-specific tyrosine-protein kinase receptor; MGC126323; MGC126324
• Gene ID: 4593
• mRNA Refseq: NM_001166280
• Protein Refseq: NP_001159752
• MIM: 601296
• UniProt ID: O15146