Recombinant Human MYL12B protein, His & T7-tagged

• Cat.No.: MYL12B-8046H
• Product Overview: Recombinant Human MYL12B aa. (Lys5~Asp172 (Accession # O14950)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.2kDa.
• Protein length: Lys5~Asp172 (Accession # O14950)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: MYL12B myosin light chain 12B [ Homo sapiens (human) ]
• Official Symbol: MYL12B
• Gene ID: 103910
• mRNA Refseq: NM_001144944.1
• Protein Refseq: NP_001138416.1
• UniProt ID: O14950