Recombinant Human MYLIP Protein (1-445 aa), GST-tagged

• Cat.No.: MYLIP-1103H
• Product Overview: Recombinant Human MYLIP Protein (1-445 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length.

Specification

• Description: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 76.9 kDa
• Protein length: 1-445 aa
• AA Sequence: MLCYVTRPDAVLMEVEVEAKANGED CLNQVCRRLGIIEVDYFGLQFTGSK GESLWLNLRNRISQQMDGLAPYRLK LRVKFFVEPHLILQEQTRHIFFLHI KEALLAGHLLCSPEQAVELSALLAQ TKFGDYNQNTAKYNYEELCAKELSS ATLNSIVAKHKELEGTSQASAEYQV LQIVSAMENYGIEWHSVRDSEGQKL LIGVGPEGISICKDDFSPINRIAYP VVQMATQSGKNVYLTVTKESGNSIV LLFKMISTRAASGLYRAITETHAFY RCDTVTSAVMMQYSRDLKGHLASLF LNENINLGKKYVFDIKRTSKEVYDH ARRALYNAGVVDLVSRNNQSPSHSP LKSSESSMNCSSCEGLSCQQTRVLQ EKLRKLKEAMLCMVCCEEEINSTFC PCGHTVCCESCAAQLQSCPVCRSRV EHVQHVYLPTHTSLLNLTVI
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: MYLIP myosin regulatory light chain interacting protein [ Homo sapiens ]
• Official Symbol: MYLIP
• Synonyms: MYLIP; IDOL; MIR
• Gene ID: 29116
• mRNA Refseq: NM_013262
• Protein Refseq: NP_037394
• MIM: 610082
• UniProt ID: Q8WY64