| Cat.No. : |
MYLK-5820H |
| Product Overview : |
Human MYLK full-length ORF ( ENSP00000355024, 1 a.a. - 992 a.a.) recombinant protein with GST-tag at N-terminal. |
| Description : |
This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3 region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq |
| Source : |
Wheat Germ |
| Species : |
Human |
| Tag : |
GST |
| Molecular Mass : |
136.6 kDa |
| AA Sequence : |
MDFRANLQRQ VKPKTVSEEE RKVHSPQQVD FRSVLAKKGT SKTPVPEKVP PPKPATPDFR SVLGGKKKLP AENGSSSAET LNAKAVESSK PLSNAQPSGP LKPVGNAKPA ETLKPMGNAK PAETLKPMGN AKPDENLKSA SKEELKKDVK NDVNCKRGHA GTTDNEKRSE SQGTAPAFKQ KLQDVHVAEG KKLLLQCQVS SDPPATIIWT LNGKTLKTTK FIILSQEGSL CSVSIEKALP EDRGLYKCVA KNDAGQAECS CQVTVDDAPA SENTKAPEMK SRRPKSSLPP VLGTESDATV KKKPAPKTPP KAAMPPQIIQ FPEDQKVRAG ESVELFGKVT GTQPITCTWM KFRKQIQESE HMKVENSENG SKLTILAARQ EHCGCYTLLV ENKLGSRQAQ VNLTVVDKPD PPAGTPCASD IRSSSLTLSW YGSSYDGGSA VQSYSIEIWD SANKTWKELA TCRSTSFNVQ DLLPDHEYKF RVRAINVYGT SEPSQESELT TVGEKPEEPK DEVEVSDDDE KEPEVDYRTV TINTEQKVSD FYDIEERLGS GKFGQVFRLV EKKTRKVWAG KFFKAYSAKE KENIRQEISI MNCLHHPKLV QCVDAFEEKA NIVMVLEIVS GGELFERIID EDFELTEREC IKYMRQISEG VEYIHKQGIV HLDLKPENIM CVNKTGTRIK LIDFGLARRL ENAGSLKVLF GTPEFVAPEV INYEPIGYAT DMWSIGVICY ILVSGLSPFM GDNDNETLAN VTSATWDFDD EAFDEISDDA KDFISNLLKK DMKNRLDCTQ CLQHPWLMKD TKNMEAKKLS KDRMKKYMAR RKWQKTGNAV RAIGRLSSMA MISGLSGRKS STGSPTSPLN AEKLESEEDV SQAFLEAVAE EKPHVKPYFS KTIRDLEVVE GSAARFDCKI EGYPDPEVVW FKDDQSIRES RHFQIDYDED GNCSLIISDV CGDDDAKYTC KAVNSLGEAT CTAELIVETM EEGEGEGEEE EE |
| Applications : |
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array |
| Notes : |
Best use within three months from the date of receipt of this protein. |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
| Quality Control Testing: : |
12.5% SDS-PAGE Stained with Coomassie Blue.
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