Recombinant Human MYOD1, His-tagged

• Cat.No.: MYOD1-165H
• Product Overview: The protein was expressed as 6*his-tagged fusion protein by E.Coli and puri?ed by Ni-sepharose. Expression Vector: PET28a.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm? tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing? sarcomas and alveolar soft part sarcomas.
• AA Sequence: 0-232aa
• Storage: The protein is stored in PBS bu?er at -20°C. Repeated freezethaw cycles should be avoided.
• Storage Buffer: The puri?ed protein was resolved in 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.0) added with 300mM Imidazole and 0.7% Sarcosyl, 15% glycerol.

Gene Information

• Gene Name: MYOD1 myogenic differentiation 1 [ Homo sapiens ]
• Official Symbol: MYOD1
• Synonyms: MYOD1; myogenic differentiation 1; MYF3, myogenic factor 3; myoblast determination protein 1; bHLHc1; MYOD; PUM; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1; MYF3
• Gene ID: 4654
• mRNA Refseq: NM_002478
• Protein Refseq: NP_002469
• MIM: 159970
• UniProt ID: P15172
• Chromosome Location: 11p15
• Pathway: C-MYB transcription factor network, organism-specific biosystem; CDO in myogenesis, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Id Signaling Pathway, organism-specific biosystem; Myogenesis, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem; Regulation of nuclear SMAD2/3 signaling, organism-specific biosystem
• Function: DNA binding; E-box binding; enzyme binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription coactivator activity; transcription factor binding; ubiquitin protein ligase binding