Active Recombinant Human NANOG protein, Arginine-tagged

• Cat.No.: NANOG-130H
• Product Overview: Recombinant human NANOG protein fused with 11 arginine domain at C-terminal, which will efficiently deliver protein intracellularly, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: Arginine
• Form: 0.50 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
• Bio-activity: DNA binding activity was demonstrated with ELISA using NANOG specific DNA binding oligo.
• AA Sequence: MASMTGGQQMGRGHHHHHHGNLYFQ GGEFSVDPACPQSLPCFEASDCKES SPMPVICGPEENYPSLQMSSAEMPH TETVSPLPSSMDLLIQDSPDSSTSP KGKQPTSAEKSVAKKEDKVPVKKQK TRTVFSSTQLCVLNDRFQRQKYLSL QQMQELSNILNLSYKQVKTWFQNQR MKSKRWQKNNWPKNSNGVTQKASAP TYPSLYSSYHQGCLVNPTGNLPMWS NQTWNNSTWSNQTQNIQSWSNHSWN TQTWCTQSWNNQAWNSPFYNCGEES LQSCMQFQPNSPASDLEAALEAAGE GLNVIQQTTRYFSTPQTMDLFLNYS MNMQPEDVESGGGGSPGRRRRRRRR RRR
• Purity: >90% by SDS-PAGE
• Applications: 1. Protein transduction for enhancing PiPS generation efficiency.2. Active protein, may be used for ELISA based DNA/Protein binding assay.3. As specific protein substrate for kinase assay.
• Storage: Keep at -20°C for long term storage. Product is stable at 4 °C for at least 7 days

Gene Information

• Gene Name: NANOG Nanog homeobox [ Homo sapiens ]
• Official Symbol: NANOG
• Synonyms: NANOG; Nanog homeobox; homeobox protein NANOG; FLJ12581; FLJ40451; hNanog; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
• Gene ID: 79923
• mRNA Refseq: NM_024865
• Protein Refseq: NP_079141
• MIM: 607937
• UniProt ID: Q9H9S0
• Chromosome Location: 12p13.31
• Pathway: Wnt Signaling Pathway and Pluripotency, organism-specific biosystem
• Function: DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity