| Description : |
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
44.5 kDa |
| AA Sequence : |
MTAEEGVQLSQQNAKDFFRVLNLNK KCDTSKHKVLVVSVCPQSLPYFAAK FNLSVTDASRRLCGFLKSLGVHYVF DTTIAADFSILESQKEFVRRYRQHS EEERTLPMLTSACPGWVRYAERVLG RPITAHLCTAKSPQQVMGSLVKDYF ARQQNLSPEKIFHVIVAPCYDKKLE ALQESLPPALHGSRGADCVLTSGEI AQIMEQGDLSVRDAAVDTLFGDLKE DKVTRHDGASSDGHLAHIFRHAAKE LFNEDVEEVTYRALRNKDFQEVTLE KNGEVVLRFAAAYGFRNIQNMILKL KKGKFPFHFVEVLACAGGCLNGRGQ AQTPDGHADKALLRQMEGIYADIPV RRPESSAHVQELYQEWLEGINSPKA REVLHTTYQSQERGTHSLDIKWSGP TRTRRLEQKLISEEDLAANDILDYK DDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
