Recombinant Human NBN Protein, His-tagged

• Cat.No.: NBN-001H
• Product Overview: Purified recombinant protein of Human nibrin (NBN), Pro461-Arg754, with N-terminal His tag, expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 461-754 a.a.
• Description: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
• Molecular Mass: 34 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol,1% Sarkosyl.

Gene Information

• Gene Name: NBN nibrin [ Homo sapiens (human) ]
• Official Symbol: NBN
• Synonyms: NBN; nibrin; ATV; NBS; P95; NBS1; AT-V1; AT-V2; nibrin; Nijmegen breakage syndrome 1 (nibrin); cell cycle regulatory protein p95; p95 protein of the MRE11/RAD50 complex
• Gene ID: 4683
• mRNA Refseq: NM_002485
• Protein Refseq: NP_002476
• MIM: 602667
• UniProt ID: O60934