Recombinant Human NCK Adaptor Protein 2

• Cat.No.: NCK2-49H
• Product Overview: Recombinant Human NCK2 is produced inE.Coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 (amino acids 119-27) protein binds human PDGFR-b (Tyr-1009); over expression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.
• Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Physical Appearance: Sterile Filtered clear solution.
• Formulation: NCK2 protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Applications: • ELISA • Inhibition Assays • Western Blotting
• Characterization: On SDS-PAGE coomassie blue stained gel, the purified recombinant protein shows a band at 45 kDa.
• Storage: Store vial at -20ºC to -80ºC. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: NCK2 NCK adaptor protein 2 [ Homo sapiens ]
• Synonyms: NCK2; NCK adaptor protein 2; GRB4; NCKbeta; SH2/SH3 adaptor protein NCK-beta; noncatalytic region of tyrosine kinase, beta; Cytoplasmic protein NCK2; Nck-2
• Gene ID: 8440
• mRNA Refseq: NM_001004720
• Protein Refseq: NP_001004720
• MIM: 604930
• UniProt ID: O43639
• Chromosome Location: 2q12
• Pathway: Axon guidance; ErbB signaling pathway; Pathogenic Escherichia coli infection-EHEC; T cell receptor signaling pathway; Axon guidance; Signaling by PDGF
• Function: Function Evidence; cytoskeletal adaptor activity; protein binding; receptor signaling complex scaffold activity