"NCL" Related Products

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Recombinant Human NCL protein, His-tagged

Cat.No.: NCL-06H
Product Overview: Recombinant Human NCL(Lys388~Gln569) fused with His tag at N-terminal was expressed in E. coli.
Description: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Source: E. coli
Species: Human
Tag: His
Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass: 26.2kDa
Endotoxin: <1.0EU per 1µg (determined by the LAL method)
Purity: > 90%
Applications: SDS-PAGE; WB; ELISA; IP.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution: Reconstitute in PBS or others.
Gene Name: NCL nucleolin [ Homo sapiens ]
Official Symbol: NCL
Synonyms: NCL; nucleolin; C23; FLJ45706; FLJ59041;
Gene ID: 4691
mRNA Refseq: NM_005381
Protein Refseq: NP_005372
MIM: 164035
UniProt ID: P19338
Chromosome Location: 2q12-qter
Pathway: Aurora B signaling, organism-specific biosystem; Pathogenic Escherichia coli infection, organism-specific biosystem; Pathogenic Escherichia coli infection, conserved biosystem; Regulation of Telomerase, organism-specific biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem; Validated targets of C-MYC transcriptional activation, organism-specific biosystem;
Function: DNA binding; RNA binding; nucleic acid binding; nucleotide binding; protein C-terminus binding; protein binding; telomeric DNA binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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