Recombinant Human NCL Protein, His/GST-tagged

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Recombinant Human NCL Protein, His/GST-tagged

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Cat.No. : NCL-442H
Product Overview : Recombinant Human NCL(Lys388~Gln569) fused with His/GST tag at N-terminal was expressed in E. coli.
Description : Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Source : E. coli
Species : Human
Tag : His/GST
Form : PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass : 26.2kDa
Protein length : Lys388~Gln569
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : > 97%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in PBS or others
Figure :

Gene Name : NCL nucleolin [ Homo sapiens ]
Official Symbol : NCL
Synonyms : NCL; nucleolin; C23; FLJ45706; FLJ59041;
Gene ID : 4691
mRNA Refseq : NM_005381
Protein Refseq : NP_005372
MIM : 164035
UniProt ID : P19338

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