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Recombinant Human NCL protein, His-tagged

Cat.No.: NCL-05H
Product Overview: Recombinant Human NCL fused with His tag was expressed in Yeast.
Description: Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Source: Yeast
Species: Human
Tag: His
Form: Lyophilized from 0.2 µm filtered PBS, pH 7.4
Molecular Mass: 54.4 kD
AA Sequence: VKLAKAGKNQ GDPKKMAPPP KEVEEDSEDE EMSEDEEDDSSGEEVVIPQK KGKKAAATSA KKVVVSPTKK VAVATPAKKAAVTPGKKAAA TPAKKTVTPA KAVTTPGKKG ATPGKALVATPGKKGAAIPA KGAKNGKNAK KEDSDEEEDD DSEEDEEDDEDEDEDEDEIE PAAMKAAAAA PASEDEDDED DEDDEDDDDDEEDDSEEEAM ETTPAKGKKA AKVVPVKAKN VAEDEDEEEDDEDEDDDDDE DDEDDDDEDD EEEEEEEEEE PVKEAPGKRKKEMAKQKAAP EAKKQKVEGT EPTTAFNLFV GNLNFNKSAPELKTGISDVF AKNDLAVVDV RIGMTRKFGY VDFESAEDLEKALELTGLKV FGNEIKLEKP KGKDSKKERD ARTLLAKNLPYKVTQDELKE VFEDAAEIRL VSKDGKSKGI AYIEFKTEADAEKTFEEKQG TEIDGRSISL YYTGEKGQNQ DYRGGKNSTWS
Storage: Long term: -80 centigrade; Short term: -20 centigrade. Avoid freeze-thaw cycles.
Gene Name: NCL nucleolin [ Homo sapiens ]
Official Symbol: NCL
Synonyms: NCL; nucleolin; C23; FLJ45706; FLJ59041;
Gene ID: 4691
mRNA Refseq: NM_005381
Protein Refseq: NP_005372
MIM: 164035
UniProt ID: P19338
Chromosome Location: 2q12-qter
Pathway: Aurora B signaling, organism-specific biosystem; Pathogenic Escherichia coli infection, organism-specific biosystem; Pathogenic Escherichia coli infection, conserved biosystem; Regulation of Telomerase, organism-specific biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem; Validated targets of C-MYC transcriptional activation, organism-specific biosystem;
Function: DNA binding; RNA binding; nucleic acid binding; nucleotide binding; protein C-terminus binding; protein binding; telomeric DNA binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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