"NCOA1" Related Products

Expand

Recombinant Human NCOA1 protein, Biotinylated

Cat.No.: NCOA1-373H
Product Overview: The His-tag biotinylated recombinant protein is purified by affinity chromatography in combination with FPLC columns. Then His-tag was removed by protease.
Description: Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivoator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family. SRC1 has been shown to be over expressed in some cancers. SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction. The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions. This protein does not contain His tag and will be useful for assays where a native untagged protein is desired. Lane 1 corresponds to 6His-tagged and biotinylated SRC-RID; Lane 2 corresponds to biotinylated SRC-RID without 6His tag.
Source: E. coli
Species: Human
Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
Molecular Mass: 19.4 kDa
Protein length: 627-786 aa
AA Sequence: SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTERHKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDHQLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT
Purity: Purified biotinylated SRC1-RID is greater than 90% homogeneous based on SDS-PAGE analysis.
Unit Definition: 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.
Applications: The biotinylated SCR1-RID (627-786) can be used for protein-protein interaction assays, particularly suitable for high throughput screening (HTS) assays.
Storage: Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles.
Gene Name: NCOA1 nuclear receptor coactivator 1 [ Homo sapiens ]
Official Symbol: NCOA1
Synonyms: NCOA1; nuclear receptor coactivator 1; bHLHe74; F SRC 1; KAT13A; NCoA 1; RIP160; SRC1; Hin-2 protein; PAX3/NCOA1 fusion protein; steroid receptor coactivator-1; renal carcinoma antigen NY-REN-52; class E basic helix-loop-helix protein 74; F-SRC-1; bHLHe42; MGC129719; MGC129720;
Gene ID: 8648
mRNA Refseq: NM_003743
Protein Refseq: NP_003734
MIM: 602691
UniProt ID: Q15788
Chromosome Location: 2p23
Pathway: Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of expression by REV-ERBA, organism-specific biosystem
Function: DNA binding; androgen receptor binding; chromatin binding; enzyme binding; histone acetyltransferase activity; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein N-terminus binding; protein binding; signal transducer activity

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.