Active Recombinant Human NCR3LG1 protein, His-tagged, Low Endotoxin

• Cat.No.: NCR3LG1-785H
• Product Overview: Recombinant Human NCR3LG1 protein(Q68D85-1)(Asp25-Ser262), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-262 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag with an affinity constant of 48.67 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.