Recombinant Human NDUFAF7 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NDUFAF7-6486H
• Product Overview: C2orf56 MS Standard C13 and N15-labeled recombinant protein (NP_653337) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes an assembly factor protein which helps in the assembly and stabilization of Complex I, a large multi-subunit enzyme in the mitochondrial respiratory chain. Complex I is involved in several physiological activities in the cell, including metabolite transport and ATP synthesis. The encoded protein is a methyltransferase which methylates Arg85 of a subunit of Complex I in the early stages of its assembly. A pseudogene related to this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 49.2 kDa
• AA Sequence: MSVLLRSGLGPLCAVARAAIPFIWR GKYFSSGNEPAENPVTPMLRHLMYK IKSTGPITVAEYMKEVLTNPAKGYY VYRDMLGEKGDFITSPEISQIFGEL LGIWFISEWMATGKSTAFQLVELGP GRGTLVGDILRVFTQLGSVLKNCDI SVHLVEVSQKLSEIQALTLTKEKVP LERNAGSPVYMKGVTKSGIPISWYR DLHDVPKGYSFYLAHEFFDVLPVHK FQKTPQGWREVFVDIDPQVSDKLRF VLAPSATPAEAFIQHDETRDHVEVC PDAGVIIEELSQRIALTGGAALVAD YGHDGTKTDTFRGFCDHKLHDVLIA PGTADLTADVDFSYLRRMAQGKVAS LGPIKQHTFLKNMGIDVRLKVLLDK SNEPSVRQQLLQGYDMLMNPKKMGE RFNFFALLPHQRLQGGRYQRNARQS KPFASVVAGFSELAWQTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NDUFAF7 NADH:ubiquinone oxidoreductase complex assembly factor 7 [ Homo sapiens (human) ]
• Official Symbol: NDUFAF7
• Synonyms: NDUFAF7; NADH:ubiquinone oxidoreductase complex assembly factor 7; MidA; C2orf56; PRO1853; protein arginine methyltransferase NDUFAF7, mitochondrial; NADH dehydrogenase (ubiquinone) complex I, assembly factor 7; NADH dehydrogenase [ubiquinone] complex I, assembly factor 7; mitochondrial dysfunction protein A homolog; protein midA homolog, mitochondrial; EC 2.1.1.320
• Gene ID: 55471
• mRNA Refseq: NM_144736
• Protein Refseq: NP_653337
• MIM: 615898
• UniProt ID: Q7L592